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ptyb12  (New England Biolabs)


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    Structured Review

    New England Biolabs ptyb12
    Reagents
    Ptyb12, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 86 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ptyb12/pTYB21+Vector/pmc11735616-26-0-2
    Average 94 stars, based on 86 article reviews
    ptyb12 - by Bioz Stars, 2026-09
    94/100 stars

    Images

    1) Product Images from "Herpesviruses mimic zygotic genome activation to promote viral replication"

    Article Title: Herpesviruses mimic zygotic genome activation to promote viral replication

    Journal: Nature Communications

    doi: 10.1038/s41467-025-55928-5

    Reagents
    Figure Legend Snippet: Reagents

    Techniques Used:

    Related Articles

    Polymerase Chain Reaction:

    Article Title: Calcium-dependent site-switching regulates expression of the atypical iam pilus locus in Vibrio vulnificus.
    Article Snippet: This article has been accepted for publication and undergone full peer review but has not been through the copyediting, typesetting, pagination and proofreading process which may lead to differences between this version and the Version of Record.. Please cite this article as doi: 10.1111/1462-2920.14763 Calcium-dependent site-switching regulates expression of the atypical iam pilus locus in Vibrio vulnificus.. Meng Pua, •, Emily Stormsb, Dan M. Chodurb and Dean A. Rowe-Magnusa,b,# aDepartment of Biology and bMolecular and Cellular Biochemistry, Indiana University Bloomington, IN #, corresponding author: Dean Rowe-Magnus, Jordan Hall, 1001 E 3rd Street, Indiana University Bloomington, IN; 812-855-8514; drowemag@indiana.edu Running Title: Calcium regulates V. vulnificus pilus expression • current address: Division of Gastroenterology and Hepatology, Mayo Clinic, Rochester MN This article is protected by copyright.

    Clone Assay:

    Article Title: Calcium-dependent site-switching regulates expression of the atypical iam pilus locus in Vibrio vulnificus.
    Article Snippet: This article has been accepted for publication and undergone full peer review but has not been through the copyediting, typesetting, pagination and proofreading process which may lead to differences between this version and the Version of Record.. Please cite this article as doi: 10.1111/1462-2920.14763 Calcium-dependent site-switching regulates expression of the atypical iam pilus locus in Vibrio vulnificus.. Meng Pua, •, Emily Stormsb, Dan M. Chodurb and Dean A. Rowe-Magnusa,b,# aDepartment of Biology and bMolecular and Cellular Biochemistry, Indiana University Bloomington, IN #, corresponding author: Dean Rowe-Magnus, Jordan Hall, 1001 E 3rd Street, Indiana University Bloomington, IN; 812-855-8514; drowemag@indiana.edu Running Title: Calcium regulates V. vulnificus pilus expression • current address: Division of Gastroenterology and Hepatology, Mayo Clinic, Rochester MN This article is protected by copyright.

    Article Title: Mucosal TLR5 activation controls healthspan and longevity
    Article Snippet: .. Briefly, to amplify each DNA fragment of FlaB and PspA, a DNA fragment containing the open reading frame of FlaB protein from Vibrio vulnificus or PspA protein from Streptococcus pneumonia were cloned into pTYB12-yielding pCMM250 or pCMM8206 (New England Biolabs, Beverly, MA, USA). .. To amplify the DNA fragments of fusion FlaB-PspA (FP), DNA fragments of FlaB (F) and PspA (P) were cloned into the pTYB12-yielding pCMM8208 (New England Biolabs).

    Article Title: The Nuclear Inclusion a (NIa) Protease of Turnip Mosaic Virus (TuMV) Cleaves Amyloid-β
    Article Snippet: .. To produce recombinant NIa protein in E. coli , the NIa gene was cloned into pTYB12 (New England BioLabs) via the EcoR I and Xho I sites. ..

    Transformation Assay:

    Article Title: Calcium-dependent site-switching regulates expression of the atypical iam pilus locus in Vibrio vulnificus.
    Article Snippet: This article has been accepted for publication and undergone full peer review but has not been through the copyediting, typesetting, pagination and proofreading process which may lead to differences between this version and the Version of Record.. Please cite this article as doi: 10.1111/1462-2920.14763 Calcium-dependent site-switching regulates expression of the atypical iam pilus locus in Vibrio vulnificus.. Meng Pua, •, Emily Stormsb, Dan M. Chodurb and Dean A. Rowe-Magnusa,b,# aDepartment of Biology and bMolecular and Cellular Biochemistry, Indiana University Bloomington, IN #, corresponding author: Dean Rowe-Magnus, Jordan Hall, 1001 E 3rd Street, Indiana University Bloomington, IN; 812-855-8514; drowemag@indiana.edu Running Title: Calcium regulates V. vulnificus pilus expression • current address: Division of Gastroenterology and Hepatology, Mayo Clinic, Rochester MN This article is protected by copyright.

    Amplification:

    Article Title: Motility Control Through an Anti-Activation Mechanism in Agrobacterium tumefaciens
    Article Snippet: .. The rem allele was amplified from a cloning construct (primer sequences in Table S4) and ligated into pTYB12 (NEB IMPACT system of protein purification), resulting in an N-terminal intein fusion to Rem. ..

    Article Title: Motility control through an anti-activation mechanism in Agrobacterium tumefaciens.
    Article Snippet: .. The rem allele was amplified from a pre- existing construct (primer sequences in Table S4) and ligated into pTYB12 (NEB IMPACT system of protein purification), resulting in an N- terminal intein fusion to Rem. ..

    Cloning:

    Article Title: Motility Control Through an Anti-Activation Mechanism in Agrobacterium tumefaciens
    Article Snippet: .. The rem allele was amplified from a cloning construct (primer sequences in Table S4) and ligated into pTYB12 (NEB IMPACT system of protein purification), resulting in an N-terminal intein fusion to Rem. ..

    Article Title: Backbone 1 H, 13 C and 15 N chemical shift assignment of full-length human uracil DNA glycosylase UNG2.
    Article Snippet: The nuclear human uracil-DNA glycosylase UNG2 is the major enzyme for removal of both misincorporated uracil (U:A) and miscoding deaminated cytosine (U:G) in the genome (Kavli et al. 2002).. In addition, UNG2 is essential in adaptive immunity where it works together with the deaminase AID in affinity maturation of antibodies (PMID:17116429).. The highly conserved catalytic C-terminal core domain of UNG (C-UNG2, residues 93–313) has previously been crystallized both as native protein and in a complex with DNA (Mol et al. 1995; Slupphaug et al. 1996).

    Construct:

    Article Title: Motility Control Through an Anti-Activation Mechanism in Agrobacterium tumefaciens
    Article Snippet: .. The rem allele was amplified from a cloning construct (primer sequences in Table S4) and ligated into pTYB12 (NEB IMPACT system of protein purification), resulting in an N-terminal intein fusion to Rem. ..

    Article Title: Motility control through an anti-activation mechanism in Agrobacterium tumefaciens.
    Article Snippet: .. The rem allele was amplified from a pre- existing construct (primer sequences in Table S4) and ligated into pTYB12 (NEB IMPACT system of protein purification), resulting in an N- terminal intein fusion to Rem. ..

    Protein Purification:

    Article Title: Motility Control Through an Anti-Activation Mechanism in Agrobacterium tumefaciens
    Article Snippet: .. The rem allele was amplified from a cloning construct (primer sequences in Table S4) and ligated into pTYB12 (NEB IMPACT system of protein purification), resulting in an N-terminal intein fusion to Rem. ..

    Article Title: Motility control through an anti-activation mechanism in Agrobacterium tumefaciens.
    Article Snippet: .. The rem allele was amplified from a pre- existing construct (primer sequences in Table S4) and ligated into pTYB12 (NEB IMPACT system of protein purification), resulting in an N- terminal intein fusion to Rem. ..

    other:

    Article Title: Herpesviruses mimic zygotic genome activation to promote viral replication
    Article Snippet: pTYB12 , NEB , N6709S , .

    Binding Assay:

    Article Title: Backbone 1 H, 13 C and 15 N chemical shift assignment of full-length human uracil DNA glycosylase UNG2.
    Article Snippet: The nuclear human uracil-DNA glycosylase UNG2 is the major enzyme for removal of both misincorporated uracil (U:A) and miscoding deaminated cytosine (U:G) in the genome (Kavli et al. 2002).. In addition, UNG2 is essential in adaptive immunity where it works together with the deaminase AID in affinity maturation of antibodies (PMID:17116429).. The highly conserved catalytic C-terminal core domain of UNG (C-UNG2, residues 93–313) has previously been crystallized both as native protein and in a complex with DNA (Mol et al. 1995; Slupphaug et al. 1996).

    Purification:

    Article Title: Backbone 1 H, 13 C and 15 N chemical shift assignment of full-length human uracil DNA glycosylase UNG2.
    Article Snippet: The nuclear human uracil-DNA glycosylase UNG2 is the major enzyme for removal of both misincorporated uracil (U:A) and miscoding deaminated cytosine (U:G) in the genome (Kavli et al. 2002).. In addition, UNG2 is essential in adaptive immunity where it works together with the deaminase AID in affinity maturation of antibodies (PMID:17116429).. The highly conserved catalytic C-terminal core domain of UNG (C-UNG2, residues 93–313) has previously been crystallized both as native protein and in a complex with DNA (Mol et al. 1995; Slupphaug et al. 1996).

    Article Title: Amyloid-beta clearance
    Article Snippet: .. Purification of the NIa Protease To produce recombinant NIa protein in E. coli, the NIa gene was cloned into pTYB12 (New England BioLabs) via the EcoRI and XhoI sites. .. The pTYB12 vector contains an N-terminal intein tag.

    Plasmid Preparation:

    Article Title: Backbone 1 H, 13 C and 15 N chemical shift assignment of full-length human uracil DNA glycosylase UNG2.
    Article Snippet: The nuclear human uracil-DNA glycosylase UNG2 is the major enzyme for removal of both misincorporated uracil (U:A) and miscoding deaminated cytosine (U:G) in the genome (Kavli et al. 2002).. In addition, UNG2 is essential in adaptive immunity where it works together with the deaminase AID in affinity maturation of antibodies (PMID:17116429).. The highly conserved catalytic C-terminal core domain of UNG (C-UNG2, residues 93–313) has previously been crystallized both as native protein and in a complex with DNA (Mol et al. 1995; Slupphaug et al. 1996).

    Recombinant:

    Article Title: The Nuclear Inclusion a (NIa) Protease of Turnip Mosaic Virus (TuMV) Cleaves Amyloid-β
    Article Snippet: .. To produce recombinant NIa protein in E. coli , the NIa gene was cloned into pTYB12 (New England BioLabs) via the EcoR I and Xho I sites. ..



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    Image Search Results


    Reagents

    Journal: Nature Communications

    Article Title: Herpesviruses mimic zygotic genome activation to promote viral replication

    doi: 10.1038/s41467-025-55928-5

    Figure Lengend Snippet: Reagents

    Article Snippet: pTYB12 , NEB , N6709S , .

    Techniques:

    Bacterial strains and plasmids used in this study

    Journal: NPJ Vaccines

    Article Title: Development of an anti-tauopathy mucosal vaccine specifically targeting pathologic conformers

    doi: 10.1038/s41541-024-00904-1

    Figure Lengend Snippet: Bacterial strains and plasmids used in this study

    Article Snippet: Subsequently, the synthesized tauRD DNA fragment was inserted into the plasmid pTYB12 (New England Biolabs) at the EcoRI-XhoI restriction sites, ensuring precise positioning for expression (Fig. and Table ).

    Techniques: Plasmid Preparation, Expressing, Clone Assay

    Strains and plasmids used in this study.

    Journal: PLoS ONE

    Article Title: Whole Genome Sequencing and Complete Genetic Analysis Reveals Novel Pathways to Glycopeptide Resistance in Staphylococcus aureus

    doi: 10.1371/journal.pone.0021577

    Figure Lengend Snippet: Strains and plasmids used in this study.

    Article Snippet: A sequenced verified spx fragment was next subcloned into E. coli expression vector pTYB12 (New England Biolabs) using Nde1-Pst1 sites, generating plasmid pAM1101.

    Techniques: Derivative Assay, Binding Assay, Plasmid Preparation, Clone Assay